A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Wankhede, Sagar B.
- Pharmacological Evaluation of Oxalis corniculata Linn for Anthelmintic Activity
Authors
1 Department of Pharmacology, Pravara Rural College of Pharmacy, Pravranagar, Maharashtra, 413736, IN
2 Dept. of Pharmaceutical Chemistry, MAEER, Maharashtra Institute of Pharmacy, Pune Maharashtra, IN
3 Department of Pharmaceutical Chemistry, Pad. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 1 (2012), Pagination: 1-4Abstract
The present study aimed at the in-vitro comparative study of anthelmintic activity of petroleum ether, ethyl acetate and methanol extracts of whole plant of Oxalis corniculata Linn. using Eisenia foetida at three different concentrations (100, 200, and 400 mg/ml) respectively. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. At the concentration of 400 mg/ml all the extracts showed very significant activities as compared to the standard drug levamisole (0.55 mg/ml). Each extract with the dose 100, 200, and 400 mg/ml, produced dose-dependent paralysis ranging from loss of motility to loss of response to external stimuli, which gradually progressed to death. In case of methanol extract which was found to be most potent, the time of paralysis and death time was observed as 11.33 and 41.33 min, respectively. In conclusion, the use of O. corniculata as possible anthelmintic is explored and further studies are suggested to isolate the active principles responsible for the activity.Keywords
Anthelmintic, Eisenia foetida, Oxalis corniculata Linn.References
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- Simultaneous Spectrophotometric Estimation of Diclofenac Sodium and Omeprazole in Capsules
Authors
1 Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-18, M.H., IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 7 (2011), Pagination: 1172-1175Abstract
Two simple, sensitive, rapid spectrophotometric methods have been developed for simultaneous estimation of diclofenac sodium (DFS) and omeprazole (OME) in capsules. First method involves solving simultaneous equations based on measurement of absorbance at two wavelengths 248.0 nm and 301.5 nm λ max of DFS and OME, respectively. Second method involves formation of Q-absorbance equation at 255.5 nm (isoabsorptive point) and 301.5 nm (λ max of OME). Beer's law was obeyed in the concentration range of 10-50 μg/ml and 5-25 μg/ml for DFS and OME, respectively. The methods were validated as per ICH guidelines. Statistical analysis proved that the methods were accurate, precise, and reproducible for analysis of DFS and OME in capsules. The wide linearity range, sensitivity, accuracy and simple procedure imply that the proposed technique demonstrated to be appropriate for routine analysis and quality control assay of capsules.
Keywords
Diclofenac Sodium, Omeprazole, Simultaneous Equation Method, Q-Absorbance Ratio Method.- UV Spectroscopic and Stability-Indicating TLC-Densitometric Method for Simultaneous Estimation of Salbutamol Sulphate and Prednisolone in Pharmaceutical Dosage Form
Authors
1 Padmashree Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 5 (2011), Pagination: 786-790Abstract
Rapid, accurate and sensitive method for simultaneous determination of salbutamol sulphate (SAL) and prednisolone (PRE) in pharmaceutical dosage form was developed in the present work. The first developed method wasArea under curve method, wavelength selected is232-222nm for salbutamol and 249-239nm for prednisolone respectively. Linearity was observed in concentration range of 6-20μg/ml for salbutamol as well as for prednisolone. Second developed method was high performance thin layer chromatography (HPTLC). The mobile phase selected was Ethyl acetate: Chloroform: methanol (6.0: 4.0:1.0 v/v/v) with UV detection at 230 nm. Retention factor for SAL and PRE were found to be 0.17 ± 0.02 and 0.52 ± 0.03 respectively. For HPTLC method, linearity was observed in the concentration range of 1-6μg/band for salbutamol and 2-6μg/band for prednisolone and drugs was subjected to oxidation, acid hydrolysis, base hydrolysis and sun light to apply stress condition for degradation studies. Results of analysis were validated statistically and by recovery studies.Keywords
Salbutamol Sulphate, Prednisolone, Area under Curve Method, TLC-Densitometry and Degradation Studies.- Development and Validation of RP-HPLC Method for Quantitative Estimation of Atorvastatin Calcium and Metoprolol Succinate in Combined Dose Capsule Formulation
Authors
1 Padmashree Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, IN
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 663-665Abstract
A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method have been developed and validated for simultaneous determination of Atorvastatin calcium and Metoprolol succinate in capsules. Chromatography was carried out by gradient technique on a reversed-phase C-18 column (4.6 mm×250 mm, 5 μm) using acetonitrile-phosphate buffer (pH 3.5)-methanol (50:40:10, v/v/v) as a mobile phase at a flow rate of 1.0 ml/min and UV detection at 276 nm. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2>0.99). The method was accurate and precise with recoveries in the range of 99.84% and 100.62% for both the drugs and relative standard deviation (R.S.D.)<2. The proposed method is highly sensitive, accurate and precise and hence was successfully applied for the reliable quantification of API content in the commercial formulation containing these drugs in combination. The different analytical performance parameters such as linearity, accuracy, precision, robustness, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines.Keywords
Atorvastatin Calcium, Metoprolol Succinate, RP-HPLC.- Spectrophotometric Methods for Simultaneous Estimation of Dexibuprofen and Paracetamol
Authors
1 Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018, Maharashtra, IN